This month sweat and tears have been my companions. Besides of dissertation writing, I was working on selection of the most favorable PEM-architecture for VEGF adsorption and endothelial cell adhesion and viability, as well as investigated in vitro bioactivity of the VEGF-functionalized biomaterial. Three different PEM films have been chosen: PLL/HA, PLL/HA cross-linked and PLL/HA with nanoparticles. Cellular response was chemo-mechano-regulated by VEGF growth factor introduction as well as NHS/EDC cross-linking and silver nanoparticles incorporation. At first VEGF adsorption efficiency and release kinetics were investigated. We were also interested in the positive effects of VEGF-functionalized PEM films on HUVECs proliferation. To complete results, chemo-mechano-regulation was applied to observe changes in pro-angiogenic factors’ secretion by endothelial cells.